CGB - Universidad Mayor

02 marzo 2020

Transient genome-wide interactions of the master transcription factor NLP7 initiate a rapid nitrogen-response cascade.

DOI : 10.1038/s41467-020-14979-6


Dynamic reprogramming of gene regulatory networks (GRNs) enables organisms to

rapidly respond to environmental perturbation. However, the underlying transient

interactions between transcription factors (TFs) and genome-wide targets

typically elude biochemical detection. Here, we capture both stable and

transient TF-target interactions genome-wide within minutes after controlled TF

nuclear import using time-series chromatin immunoprecipitation (ChIP-seq) and/or

DNA adenine methyltransferase identification (DamID-seq). The transient

TF-target interactions captured uncover the early mode-of-action of NIN-LIKE

PROTEIN 7 (NLP7), a master regulator of the nitrogen signaling pathway in

plants. These transient NLP7 targets captured in root cells using temporal TF

perturbation account for 50% of NLP7-regulated genes not detectably bound by

NLP7 in planta. Rapid and transient NLP7 binding activates early nitrogen

response TFs, which we validate to amplify the NLP7-initiated transcriptional

cascade. Our approaches to capture transient TF-target interactions genome-wide

can be applied to validate dynamic GRN models for any pathway or organism of


Investigadores Participantes del Centro



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