02 marzo 2020

Transient genome-wide interactions of the master transcription factor NLP7 initiate a rapid nitrogen-response cascade.

DOI : 10.1038/s41467-020-14979-6

DESCARGAR
José Miguel Álvarez

Dynamic reprogramming of gene regulatory networks (GRNs) enables organisms to

rapidly respond to environmental perturbation. However, the underlying transient

interactions between transcription factors (TFs) and genome-wide targets

typically elude biochemical detection. Here, we capture both stable and

transient TF-target interactions genome-wide within minutes after controlled TF

nuclear import using time-series chromatin immunoprecipitation (ChIP-seq) and/or

DNA adenine methyltransferase identification (DamID-seq). The transient

TF-target interactions captured uncover the early mode-of-action of NIN-LIKE

PROTEIN 7 (NLP7), a master regulator of the nitrogen signaling pathway in

plants. These transient NLP7 targets captured in root cells using temporal TF

perturbation account for 50% of NLP7-regulated genes not detectably bound by

NLP7 in planta. Rapid and transient NLP7 binding activates early nitrogen

response TFs, which we validate to amplify the NLP7-initiated transcriptional

cascade. Our approaches to capture transient TF-target interactions genome-wide

can be applied to validate dynamic GRN models for any pathway or organism of

interest.

Investigadores Participantes del Centro

Laboratorio de Regulación del Genoma Vegetal

Doctor en Ciencias Biológicas, Pontificia Universidad Católica de Chile


LEER MÁS
Edificio2

Contacto

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