03 agosto 2016

High quality RNA extraction from Maqui berry for its application in next-generation sequencing.

DOI : 10.1186/s40064-016-2906-x

DESCARGAR
Carolina Sánchez

Maqui berry (Aristotelia chilensis) is a native Chilean species that produces

berries that are exceptionally rich in anthocyanins and natural antioxidants.

These natural compounds provide an array of health benefits for humans, making

them very desirable in a fruit. At the same time, these substances also

interfere with nucleic acid preparations, making RNA extraction from Maqui berry

a major challenge. Our group established a method for RNA extraction of Maqui

berry with a high quality RNA (good purity, good integrity and higher yield).

This procedure is based on the adapted CTAB method using high concentrations of

PVP (4 %) and β-mercaptoethanol (4 %) and spermidine in the extraction buffer.

These reagents help to remove contaminants such as polysaccharides, proteins,

phenols and also prevent the oxidation of phenolic compounds. The high quality

of RNA isolated through this method allowed its uses with success in molecular

applications for this endemic Chilean fruit, such as differential expression

analysis of RNA-Seq data using next generation sequencing (NGS). Furthermore, we

consider that our method could potentially be used for other plant species with

extremely high levels of antioxidants and anthocyanins.

Investigadores Participantes del Centro

Advanced Genomics Laboratory

Doctora en Biotecnología, Universidad Técnica Federico Santa María y Pontificia Universidad Católica de Valparaíso, Chile.


LEER MÁS
Edificio2

Contacto

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